Imikhiqizo
Amapayipi; Case No.: 5625-37-6
Izimpawu:I-1,4-Piperazediditiedineitane sulfonic acid;
I-1,4-Piperazeditiethanuesulphonic acid;
I-1,4-pipererazineethanulfonate; piperazine-n, N'-BIS (2-Ethanusulfon acid);
piperazine-n, N'-BIS (2-ethanusulfon acid), usawoti we-sodium; amapayipi
Impahla yamakhemikhali yamapayipi
● Ukubukeka / Umbala: I-White / Sula Crystalline Powder
● Iphuzu elincibilikayo:> 300 ° C (lit.)
● Inkomba ebonakalayo: 1.6300 (ukulinganisa)
● I-PKA: 6.8 (ngo-25 ℃)
● PSA: 131.98000
● Ubuningi: 1.505 g / cm3
● logp: 0.41700
● Isitoreji se-temp.:store ku-rt.
● I-Solubity.:0.1 M Naoh: 0.25 g / ml, ecacile, engenamibala
● I-SOLUBLICISITILY.IBHODI ELINGCWELE.
● xLogp3: -6.7
● I-Hydrogen Bond Donor Count: 2
● Isibalo sakwama-Hydrogen Bond samukelwa: 8
● I-Rotatable Bond Count: 6
● Isisindo esiqondile: 302.06062865
● Isibalo se-athomu esindayo: 18
● Ukuba yinkimbinkimbi: 397
Ubumsulwa / ikhwalithi
99% * Idatha evela kubahlinzeki be-raw
I-1,4-piperazinebis (ethanulfonicacid) 99% * Idatha evela kubahlinzeki be-reagent
Imininingwane Ephelile
● pictograph (s):
Xi
● Amakhodi Ayingozi: XI
● Izitatimende: 36/37/38
● Izitatimende Zokuphepha: 22-24/15-37 / 39-26
-Sizayo
Amakilasi Amakhemikhali:Okunye ukusetshenziswa -> ama-buffers e-biological
Ukumamatheka kwe-canonical:C1cn (CCN1CCS (= o) (= o) o) CCS (= o) (= o) o
Sebenzisa:I-Piperazine-N, N'-BIS- (2-EthanesULconic Acid) Ultra -Intu> 99.5%) i-Intermedia ebalulekile yamakhemikhali kuzo zonke izinhlobo zamakhemikhali amakhemikhali. I-1,4-Piperazenitietiethanuulfonic acid, i-piperazine-n, N'-BIS- (2-ethanusulfon acid) (amapayipi ayi-2 I-ejenti ebuhlungu ene-PKA eduzane ne-Ph.Pipes Ph.Pipes [Piperazine-N, N'-BIS (2-EthanesOnic Acid) isetshenziswa kaningi njenge-ejenti ebuhlungu e-biochemistry. Kuyinto i-ethanesulfonic acid buffer ethuthukiswe yi-Good et al. Ngawo-1960s. Amapayipi ane-PKA eduze kwe-PhIOLioliological Ph eyenza kube lusizo kumsebenzi wesiko leselula. Kubhalwe phansi ukunciphisa ukulahleka kwe-lipid lapho kubhebhethekisa i-glutaraldehdehyde Histology kuzicubu zezitshalo kanye nezilwane.Izindlela ezinemininingwane etholakalayo: amapayipi, usawoti weSesquisodium; Amapayipi anosawoti we-disotassium; Amapayipi usawoti; Amapayipi, usawoti we-sodium. Ama-Buffers angalungiswa ngokungeza isixazululo sesisekelo kumapayipi samahhala, akhiphe i-PH efanelekile, noma ngokuxuba izixazululo zokulinganisa kukasawoti we-monosodium kanye nosawoti we-disedium, okuthethelele ku-pH efanelekile. Amapayipi, asetshenziswa i-ejenti yokuhlasela e-Biochemistry. Amapayipi ane-PKA (6.76 ku-25 ° C) eduze kwe-pH phy yenza kube lusizo kumsebenzi wesiko leselula. Kubikwe amaphrothokholi ekusebenziseni amapayipi okuhlukaniswa kwe-Gyoxylated RNA ngama-agarose gels e-Agarose, e-Nuclease S1 emugqeni ye-RNA, nasekuvikelweni kweRibonucLuase Ukuvikelwa Kwama-Assay Protocols. Kusetshenziswe amapayipi njenge-buffer ku-Glutaraldehyde Ukulungiswa kwamasampula wezicubu. Kunconywe amapayipi okusetshenziswa kuma-buffers esifundweni se-vitro se-caspases 3, 6, 7, kanye no-8. Ucwaningo olushicilelwe lubonise usizo lwamapayipi njenge-anfer ye-Ion Kusetshenziswe amapayipi kusiko leselula ukuthola okunjalo njengobunjiniyela beprotheni ye-membrane eguqukayo e-Escherhia Coli.
Isingeniso Esinemininingwane
Amapayipi akhululekile acid, abizwa nangokuthi njenge-1,4-Piperazeditietiethanuesulfonican acid, kuyinto enhle ye-buffer Okuphawuleka ngokuba nenani le-Pka elifana ne-pH ajwayelekile ngokomzimba. Njengoba kunje, amapayipi amahhala acid asetshenziswa kaningi njenge-buffer ku-Biochemical Research.Pipes yi-zwitterionionic, piperazic buffer ewusizo kuhlu lwe-pH lungu-6.1 - 7.5. Amapayipi angenawo amandla okwenza inqwaba ebalulekile enama-ion amaningi wensimbi futhi inconyelwe ukusetshenziswa njenge-buffer engahlanganisi ngezixazululo ezinama-ironis ngensimbi. Amapayipi anezinhlobonhlobo ezahlukahlukene zezicelo futhi asetshenziswa kakhulu kwabezindaba zama-cell Culture, ku-Pretein Crystallization, njenge-buffer esebenzayo ku-Electrophoresis, futhi njenge-exent e-Isoelectric egxile kanye ne-chromatography. Lesi buffer siyakwazi ukwakha ama-radicals futhi ngenxa yalokho asilungele ukusabela kabusha kwe-redox. Ilungele ukusetshenziswa ku-bicinchoninic acid (BCA) Assay. I-solubity yamapayipi iyanda lapho i-acid yamahhala iguqulwa ibe usawoti we-sodium.Buffers yenziwa ngokungeza isixazululo sesisekelo kumapayipi mahhala amapayipi amapayipi futhi akhiphe ku-pH oyifunayo
Amapayipi ayilungu le-ethanesulfonic acid Buffer Buffer, eyethulwa okokuqala nge-ET et al. Njengoba i-Pka yayo ku-37 ° C iseduze kwe-pH php, amapayipi anezicelo emsebenzini wesiko leselula.
Isingeniso Esinemininingwane
Amapayipi, abizwa nangokuthi i-piperazine-n, N'-BIS (2-ethanesfoc acid), i-buffer evame ukusetshenziswa ocwaningweni lwebhayoloji kanye ne-biochemical. Yikhompiyutha ye-zwitterionic esisiza ukugcina i-PH ezinzile ezixazululweni zamanzi.
Enye yezicelo eziyisisekelo zamapayipi zisemkhakheni we-biology molecular kanye ne-biochemistry, lapho isetshenziswa khona njenge-buffer yokulungiselela kanye nokugcinwa kwamasampula endalo. Amapayipi anenani le-PKA elicishe libe ngu-7.46, okwenza kube yinto elungele ukulondolozela uhla lwe-6.7 kuye ku-7.9 kumazinga okushisa angokomzimba (cishe ama-37 degrees Celsius).
Amapayipi asetshenziswa kakhulu ekugcineni kokugcina i-ph ngesikhathi sezinqubo ezinjengokuhlanzwa kwamaprotheni, amehlo enzyme, kanye ne-gel electrophoresores. Kuyasiza ukunciphisa izinguquko ze-PH ezingathinta ukwakheka kanye nokusebenza kwamaprotheni nama-enzyme.
Okunye ukusetshenziswa kwamapayipi ku-Cell Culture Research. Isetshenziswa kakhulu njenge-buffer yemidiya yesiko yeseli. Amapayipi asiza ukugcina i-PH eqhubekayo ekwakheni, okubaluleke kakhulu ekukhuleni nasekusebenzeni kwamaseli. Inikeza nemvelo ezinzile zokutadisha izinqubo zeselula nokwenza izivivinyo.
Amapayipi asetshenziswa futhi kumasu ahlukahlukene wokuhlaziya, njenge-chromatography ewuketshezi kanye ne-capillary electrophoresis. Umthamo wayo wokubuphazamisa kanye nokuqina kwe-PH kwenza kube lula ukusetshenziswa kulezi zinqubo, lapho kubalulekile khona ukulawulwa kwe-PH imiphumela efanele.
Ngokwengeziwe, amapayipi athole izinhlelo zokusebenza ekwakhekeni kwezidakamizwa kanye nezinhlelo zokulethwa. Ingasetshenziswa njenge-buffer ezakhiweni ezenza imithi zokwandisa ukuzinza kwezidakamizwa futhi ithuthukise ukumuncwa kwezidakamizwa.
Sekukonke, amapayipi yi-ejenti eliguquguqukayo eliguquguqukayo elinezicelo ezahlukahlukene ku-biological and biochemical ucwaningo, amasiko weseli, amasu wokuhlaziya, kanye nokwakheka komakhemikhali. Amandla awo okugcina i-ph ezinzile kwenza kube yithuluzi elibalulekile emasimini ahlukahlukene esayensi.
