Imikhiqizo
I-mopso sodium usawoti; Case No.: 79803-73-9
Amanyanjana: 4-morpholinepropasunulfonicacid, b-hydroxy-, usawoti we-monosodium (9ci)
Impahla yamakhemikhali ye-mopso sodium usawoti
● I-PKA: 6.9 (ngo-25 ℃)
● Psa:98.28000
● logp: -0.75660
● Isitoreji se-temp.:store ku-rt.
● I-Solubity.Ke: 1 m ku-20 ° C, icacile, ingenamibala
Imininingwane Ephelile
● pictograph (s):
Xi
● Amakhodi Ayingozi: XI
● Izitatimende: 36/37/38
● Izitatimende zokuphepha: 26-36
-Sizayo
Sebenzisa:I-Mopso Sodium yi-buffer yemvelo futhi ibizwa ngokuthi isizukulwane sesibili "sakahle" buffer esibonisa i-solubity ethuthukisiwe ngokuqhathaniswa ne-buffers yendabuko ye- "Good". I-PKA ye-Mopso Sodium ingu-6.9 okwenza kube ngumuntu ofanele okhethwayo wezakhi ze-buffer ezidinga i-pH kancane ngezansi ngokomzimba ukuze zilondoloze indawo ezinzile kusixazululo. I-Mopso Sodium ibhekwa njenge-non-anobuthi emigqeni yamaseli futhi ihlinzeka nge-sodium yesixazululo esiphezulu.Ukulungiswa kwe-s cell Culte Media, ukwakheka kwe-biopharmaceutical Buffer (zombili i-Upstream and Dowstream) kanye ne-DiagnsTream) ne-DiagnsTream. Ama-MOPSO asuselwa kwi-MOPSO achazwe ukuze alungiswe amaseli kusuka kumasampula omchamo.
Isingeniso Esinemininingwane
Mopso sodium usawoti, eyaziwa nangokuthi i-sodium 3- (N-Morpholino) Propanesulfonate, iyisidina esivame ukusetshenziswa ocwaningweni lwebhayoloji kanye ne-biochemical. Kuyimpuphu we-crystalline emhlophe enyakazisa kakhulu emanzini. Usawoti we-sodium we-mopso uvame ukusetshenziswa njenge-buffer ukuze ulondoloze inani elizinzile le-PH ekuhlolweni okuhlukahlukene kwebhayoloji kanye nokuphendula kwe-enzymatic. Kuwusizo kakhulu kwizicelo ezidinga uhla lwe-pH olungu-6.5 kuye ku-7.9 ngenxa yenani layo le-PKA elingu-7.2. Leli banga le-buffer lenza ukuthi lilungele isiko leseli, ukuhlanzwa kwamaprotheni, kanye namasu we-molecular biology.
Ngaphezu komthamo wayo wokubukelwa, usawoti we-mopso sodium futhi unamandla okuqinisa amaprotheni athile nama-enzyme, asiza ukugcina umsebenzi wawo nesakhiwo. Kubhekwa njenge-buffer ye-zwitterionicic, okusho ukuthi kungenzeka kube khona ngamafomu afanele nangokungezinhle, ngokuya nge-PH yesisombululo. Lapho usebenzisa usawoti we-sodium we-mopso, kubalulekile ukukala futhi ulungiselele izixazululo ze-buffer ngokunemba ukufezekisa izinga le-PH oyifunayo. Inkomba ye-PH kaPh ye-PH noma i-PH iyanconywa ukuqapha futhi ilungise i-PH ngokufanele.
Sekukonke, i-mopso sodium usawoti iyithuluzi elibalulekile ekucwaningweni kwelebhu, ukuhlinzeka ngemvelo ezinzile kanye nokusekela izivivinyo ezahlukahlukene zebhayoloji kanye ne-biochemical.
Isicelo
I-Mopso Sodium Salt (3- (N-Morpholino) Propanesulfonic acid solium usawoti) inezicelo eziningana eziwusizo, ikakhulukazi emkhakheni we-biochemistry kanye ne-biotechnology. Nazi izindlela usawoti we-mopso sodium angazuzisa ngazo:
Umenzeli we-Buffering:Usawoti we-sodium we-mopso uvame ukusetshenziswa njenge-ejenti ebukhali ekuhlolweni okuhlukahlukene kwebhayoloji kanye ne-biochemical. Kuyasiza ukugcina ibanga elizinzile PH, elibalulekile ngomsebenzi ofanele we-enzyme, ukuqina kwamaprotheni nezinye izinqubo zebhayoloji.
I-Protein Crystallization:Usawoti we-sodium we-mopso uvame ukusetshenziswa ezikrinini ze-protein crystallization ukwenza lula ukukhula kwamakristalu aphezulu amaprotheni. Umthamo wayo wokubuphazamisa usiza ukugcina i-PH engaguquki, ebalulekile ukuze akwazi ukuqina kanye nokwakheka kwamakristalu amaprotheni.
I-electrophoresis:Usawoti we-sodium we-mopso usebenza njengengxenye yokuhlanza amasu afana ne-SDS-Page (i-sodium dodecyl sulfate-polylacrylel sulfate-polynacryl sulfate-polyloko aulfate-polycoremide gel electophoresis) esetshenziselwa ukuhlaziya amaprotheni. Izakhiwo zayo ezibucayi zisiza ukugcina i-ph ezinzile, ukuqinisekisa ukwahlukana kwamaprotheni nokuhlaziywa.
I-Enzyme Abs:Usawoti we-sodium we-mopso ulusizo ezifundweni ze-enzyme nezifundo ze-kinetic, lapho kusiza khona ukugcina imvelo ezinzile futhi ezilawulwa yi-PH. Lokhu kubalulekile ekulinganiseni okunembile komsebenzi we-enzyme nokuqonda amapharamitha we-kinetic.
Izisombululo ze-Biochemical:I-Mopso sodium usawoti isetshenziswa ekwakhiweni kwezixazululo ze-biochemical, njenge-buffers yokukhishwa kwamaprotheni, ukuhlanzwa, nokugcinwa. Umthamo wayo wokuhlanza uqinisekisa ukuthi i-PH ihlala ihlala njalo, inciphisa ukuhlukahluka ekuziphatheni kwamaphrotheni nokusebenza.
Kubalulekile ukuqaphela ukuthi ukugxila okuqondile kanye nemibandela ye-mopso sodium usawoti ukusetshenziswa kungahluka ngokuya ngokuhlola okuthile noma isicelo. Njalo bhekisa kumaphrothokholi afanele kanye nemihlahlandlela yokulungiselela okufanelekile nokusetshenziswa kosawoti we-mopso sodium kuzivivinyo zakho.
