Imikhiqizo
Mopso; Case No.: 68399-77-9
Amanyanjana: 3-morpholino-2-hydroxypropanesfulfonic acid; 3-morpholino-2hopsa
Impahla yamakhemikhali ye-mopso
● Ukubukeka / Umbala: I-White Crystalline Powder
● Iphuzu elincibilikayo: 275-280 ° C (DECE.)
● Inkomba evulekayo: 1.539
● I-PKA: PK1: 6.75 (37 ° C)
● Psa:95.45000
● Hlensity: 1.416 g / cm3
● Logp: -0.41400
● Isitoreji se-temp.:store ku-rt.
● I-Solubity.:HO: 0.5 m ku-20 ° C, kucacile
● I-SOLUBLITILY.:Water Solubity ngaphansi kwezimo ezifiselekayo ca.112,6 g / l ku-20 ° C.
● xLogpp3: -4.1
● I-Hydrogen Bond Donor Count: 2
● Isibalo sakwamandla e-hydrogen
● Ukulondolozwa kwebhondi okujikelezayo: 4
● Isisindo esiqondile: 225.06709375
● Isibalo se-athomu esindayo: 14
● Ukuba yinkimbinkimbi: 254
Imininingwane Ephelile
● pictograph (s):
Xi
● Amakhodi Ayingozi: XI
● Izitatimende: 36/37/38
● Izitatimende Zokuphepha: 26-36-37 / 39
-Sizayo
Amakilasi Amakhemikhali:Ama-Nitrogen Compounds -> Morpholine
Ukumamatheka kwe-canonical:C1coccn1cc (cs (= o) (= o) o) o
Sebenzisa:I-MOPSO iyi-buffer esebenza ebangeni le-6-7 Ph. Isetshenziswe ekuhlolweni kwemithi. I-MOPSO iyi-buffer yemvelo futhi ibizwa ngokuthi isizukulwane sesibili "i-buffer enhle" ekhombisa i-soublity ethuthukisiwe ngokuqhathaniswa ne-buffers yendabuko ye- "Good". I-PKA ye-MOPSO ingu-6.9 okwenza kube ngumuntu ofanele okhethwayo wezakhi ze-buffer ezidinga i-pH kancane ngezansi ngokomzimba ukuze zilondoloze indawo ezinzile kusixazululo. I-MOPSO ibhekwa njenge-non-anobuthi emigqeni yamaseli futhi ihlinzeka ngezixazululo eziphezulu.MOPSO ingasetshenziswa emithonjeni yezeziko zamaselula, ukwakheka kwe-biopharmaceutical Buffer (zombili i-Upstream and Downtstream) ne-Diagnstream) kanye ne-DiagnsTream.
Isingeniso Esinemininingwane
I-Mopso (3- (N-Morpholino) PropasulfonicFonic Acid) i-Commin Compound engokwesigaba se-sulfonic acids. Nawa amanye amaphuzu asemqoka nge-mopso:
Isakhiwo samakhemikhali:I-Mopso inefomula yamakhemikhali C7H17NO4S futhi iqukethe indandatho ye-morpholine (i-heterocyclic compound) enamathiselwe ecaleni le-propane ngeqembu le-sulfonic acid (SO3H).
Izakhiwo ze-Buffer:I-Mopso isetshenziswa kakhulu njenge-buffer ku-biochemistry kanye nokuhlolwa kwe-molecular biology. Inenani le-PKA elicishe libe ngu-7.20 ngo-25 ° C, okwenza lilungele ukuphathwa ngaphakathi kobubanzi be-PH ka-6.2 kuye ku-7.6.
Umthamo we-Buffer:I-MOPSO inesilinganiso esilinganiselwe sokuhlasela ngaphakathi kwebanga layo elisebenzayo le-PH. Kungasiza ukugcina i-PH ezinzile ezinhlelweni ezahlukahlukene zebhayoloji kanye namakhemikhali, ukuvimbela ukuguquguquka okukhulu okungathinta umsebenzi wama-enzyme noma ukuqina kwama-molecule.
Izicelo zebhayoloji: I-MOPSO ivame ukusetshenziselwa isiko leseli, ukuhlanzwa kwamaprotheni, nezinye izivivinyo zebhayoloji ezidinga ibanga elithile le-PH. Kuwusizo ikakhulukazi ezimweni lapho i-PH idinga ukugcinwa eduze kwezimo zomzimba.
Ukuqina kwamakhemikhali: I-Mopso iqinile ngamakhemikhali futhi ayinciki kalula ngaphansi kwezimo ezijwayelekile zokugcina. Kodwa-ke, kubalulekile ukuyigcina endaweni eyomile futhi epholile ukuvikela ukumuncwa komswakama nokuwohloka okungaba khona.
Ukucatshangelwa Kwezokuphepha:I-Mopso ngokuvamile ithathwa njengephephile ukuze isetshenziswe kuzilungiselelo zelebhu uma iphathwe kahle. Kodwa-ke, njenganoma imuphi amakhemikhali, kubalulekile ukulandela amaphrothokholi okuphepha afanele, njengokugqoka imishini yokuvikela efanele (amagilavu, ama-goggles) futhi usingatha endaweni enomoya omuhle.
Kuyaqapheleka ukuthi ukusetshenziswa okuthile nokuhlushwa kwe-mopso kungahluka ngokuya ngezidingo zokuhlola. Njalo bhekisa ekhasini ledatha yezobuchwepheshe yomkhiqizo noma uthintane nochwepheshe onolwazi ngemiyalo enemininingwane kanye nokucatshangelwa okuthile kokuhlolwa kwakho.
Isicelo
I-Mopso inezinhlobonhlobo zezicelo ezinkundleni ezahlukahlukene zesayensi. Nazi ezinye izinhlelo ezivamile ze-mopso:
Umenzeli we-Buffering:I-MOPSO isetshenziswa kakhulu njenge-ejenti ebukhali ekucwaningweni kwemvelo kanye ne-biochemical. Kuyasiza ukugcina i-PH ehlala njalo izixazululo, okubalulekile ekugcineni umsebenzi nokuqina kwama-enzyme, amaprotheni, namanye ama-biomolecule.
Isiko Leselula:I-MOPSO isetshenziswa kwabezindaba zesiko leselula ukuthi balondoloze ibanga elithile le-PH (ezungeze i-PH 7.2) elilungele ukukhula kanye nokugcinwa kwemigqa ethile yeseli.
Ukuhlanzwa kwamaphrotheni:I-MOPSO ingasetshenziswa njenge-buffer ezinhlanganweni zokuhlanza ama-protein, lapho isiza khona ukugcina ukuqina kanye nomsebenzi wamaprotheni ngezinyathelo ezahlukahlukene zokuhlanzwa.
Izifundo ze-Enzyme:I-Mopso isetshenziswa kaningi njenge-buffer ezifundweni ze-enzymatic ukuze ilondoloze i-pH efanelekile yomsebenzi we-enzyme. Kuwusizo ikakhulukazi ekutadisheni ama-enzyme asebenza kahle ngakuPh 7.
I-electrophoresis:I-MOPSO ingasetshenziswa njenge-buffer kumasu we-electrophoresis, njenge-SDS-Page (Sodium Dodecyl Sulfate-PolyAmide Gel Electrophoresis), ukuhlinzeka ngobubanzi be-PH bokuhlukaniswa kwamaphrotheni nokuhlaziywa.
Ukwakhiwa kwezidakamizwa:I-MOPSO isetshenziswa ekwakhiweni kwezidakamizwa ezithile, ikakhulukazi lezo ezidinga ukuqina kwe-PH ukuqina nokusebenza ngempumelelo.
Synthesis yamakhemikhali: I-MOPSO ingaqashwa njenge-realent noma i-catalyst ekuphendukeni okuthile kwamakhemikhali kanye nezinqubo zokuhlobana.
Kubalulekile ukuqaphela ukuthi ukugxila okuqondile nokusetshenziswa kwe-mopso kungahluka ngokuya ngohlelo lokusebenza nezidingo zokuhlola. Hlala uthintana nezincwadi noma ulandele imihlahlandlela enikezwe abakhiqizi noma abacwaningi abatholakele emkhakheni othize wokufunda.
