Imikhiqizo
Mops sodium usawoti; I-Case No.: 71119-22-7
Amanyanjana: 71119-22-7; mops sodium usawoti; i-sodium 3-morpholinopropasesulfonate; mops-na; 4-morpholinepropasusulfonic acid acid acid, ama-sodium acid; usawoti); i-sodium 3-morpholin-4-ylpropane-1-sulfonate; 3- (4-morpholinopropane propasulfonicas usawoti we-sodium; i-sodium 3-morpholinopropane-1-sulfonate-1-sulfonate-1- (4-morphonate; 3- (4-Morpholino) Propase Sulfonic, Sodium Usawoti; I-sodium; 3- (morpholin-4-yl) propane-1-sulfonate; c7h15no4s.na; Schain161682; dtxsid30772246; c7-h15-n-'-0859a ; Akos015897419; Akos015964205; Akos024306967; AC-24632; AS-14495; I-SY0616888; I-SY061838; I-acid sodium usawoti; cs-0120956; ft-0613841; m0713841; I-sodium 3- (4
Impahla yamakhemikhali ye-mops sodium usawoti
● Ukubukeka / umbala: i-powder emhlophe
● iphuzu elincibilikayo: 277-282 ° C
● I-PKA: 7.2 (ngo-25 ℃)
● Psa:I-78.05000
● Hlensela: 1.41 [ngo-20 ℃]
● logp: 0.27260
● Isitoreji se-temp.:store ku-rt.
● I-Solubity.Ke: 1 m ku-20 ° C, icacile, ingenamibala
● I-allubility yamanzi.-SOLUBE NGAMANZI (523 G / L ku-20 ° C).
● I-Hydrogen Bond Donor Count: 0
● Isibalo sakwamandla e-hydrogen
● Ukulondolozwa kwebhondi okujikelezayo: 4
● Isisindo esiqondile: 231.05412338
● Isibalo se-athomu esindayo: 14
● Ukuxaka: 233
Imininingwane Ephelile
● pictograph (s):
Xi
● Amakhodi Ayingozi: XI
● Izitatimende: 36/37/38
● Izitatimende Zokuphepha: 24/15-36-26
-Sizayo
Ukumamatheka kwe-canonical:C1coccn1cccs (= o) (= o) [o -]. [Na +]
Sebenzisa:I-MOPS sodium usawoti yi-ejenti ebukhali esetshenziswa kumakhemikhali we-organic.
Isingeniso Esinemininingwane
Mops sodium usawotiIngabe i-ejenti esetshenzisiwe esetshenziswa e-biochemistry kanye ne-molecular biology eyakhethwa futhi echazwe yi-ET et al.
Kuyinto i-zwitterionionic, i-morpholinic buffer ewusizo kuhlu lwe-pH olungu-6.5 - 7.9 futhi oluvame ukusetshenziswa esetshenziselwa ama-s cell Culte Media, njengesixhumanisi esisebenzayo ku-electrophoresis kanye nokuhlanzwa kwamaprotheni ku-chromatography.
Ama-MOPs awenzanga amandla okwenza inkimbinkimbi nama-ion amaningi futhi anconyelwe ukusetshenziswa njenge-buffer engahlanganisi ngezixazululo ezinama-ironis ama-ion. I-MOPS ivame ukusetshenziswa emithonjeni yakwamasiko ebekiwe yamagciwane, imvubelo namaseli e-mammalian. I-MOPS ibhekwa njenge-buffer enhle kakhulu yokusebenzisa ukuhlukanisa i-RNA ngama-agarose gels. Kunconywa ukuthi asuse ama-mops ama-buffers nge-filtration kunokuba abe ne-autoclave ngenxa yobunikazi obungaziwa ngemikhiqizo yokonakalisa okuphuzi okwenzeka ngemuva kwe-mops nge-autoclave. Ilungele ukusetshenziswa ku-bicinchoninic acid (BCA) Assay.
I-Mops sodium usawoti ingaxutshwa nama-mops mahhala i-acid ukuze uthole i-pH efiselekayo. Ngenye indlela, mops free acid ingathunyelwa nge-sodium hydroxide ukuthola i-pH PH.
Isicelo
I-Mops sodium usawoti, owaziwa nangokuthi usawoti we-3- (n
Enye yezicelo eziphambili zikasawoti we-sodium we-mops kumasiko weseli kanye nokwakheka kwemidiya.Imvamisa isetshenziswa njenge-ejenti ebukhali kwabezindaba zamaseli ukuze balondoloze i-pH eqhubekayo futhi banikeze indawo eqinile yokukhula kwamaseli nokwanda. I-MOPS Buffering isetshenziselwa kakhulu izinhlelo zesiko laseMammalian Cell.
I-Mops sodium usawoti nayo isetshenziswa ku-DNA ne-RNA GEL Electrophoresis. Isebenza njenge-buffer esebenzayo ukuqinisekisa i-ph eqinile inqubo ye-electrophoresis. Kuyasiza ukugcina ubuqotho nokuqina kwama-nucleic acid ngesikhathi sokuhlukanisa nokuhlaziywa yi-gel electophoresores.
Ngaphezu kwalokho, usawoti we-sodium we-mops usetshenziswa kuma-protein ucwaningo kanye namasu wokuhlaziya, njenge-SDS-Page (Sodium Dodecyl Sulfate-Polylacrylamide Gel Electrophoresis). Isetshenziswa kakhulu njengengxenye ye-buffer yesampula kulungiselelo lesampula le-protein, ukusiza ukusola kanye namaprotheni edevu ngempumelelo.
Ezisetshenzisweni ze-molecular Biology, usawoti we-sodium we-moss uvame ukusetshenziswa ku-PCR (Polymerase Chain Reaction) namanye amasu wokukhuphula ama-DNA.Umthamo wayo wokuhlanza isiza ukugcina i-PH ekwahlu oluphelele lomsebenzi we-DNA Polymerases namanye ama-enzyme ahilelekile kwinqubo yokukhuphula.
I-Mops sodium usawoti nayo ingasetshenziswa njenge-buffer ezindleleni ezahlukahlukene ze-biochemical amehlo, izifundo ze-enzyme kinetics, nezinqubo zokuhlanza amaprotheni.Amandla awo okugcina izinga elizinzile le-PH likwenza lube yigugu ekugcineni umsebenzi nokuqina kwama-enzyme namaprotheni ngalezi zinqubo zokuhlola.
Kubalulekile ukulandela imihlahlandlela enconyiwe yokulungiselela kanye nokusetshenziswa kosawoti we-sodium mops ukuqinisekisa imiphumela efanelekile kanye nokuvela kabusha kwezivivinyo. Ngaphezu kwalokho, kubalulekile ukugcina i-reagent kahle futhi uvikele ukungcoliswa ukuze kugcinwe ukusebenza kwayo.
