Imikhiqizo
Hepps; Case No.: 16052-06-5
Amanyanjana: Hepps; 16052-06-5; I-EPPS; I-acid; 1-piperazinepropasusulfonic acid, 4- (2-hydroxyethyl) -; i-acid); 3957385; 8JN5pqw99c; 4- (2-hydroxyethyl) -1-piperazinepropane sulfonic; 1-piperazinepropane sulfonic; I-acid; EP1; N-2-HYDROXYYYLYPINERAZIne-N'-8-8JN5PQW9CC; STXSID98490; DTXSID98490; I-DTXSID9840; I-EPPS, 49 (Ukuthathwa); I-HY-D0873; C9-H20-N2-O4-O4-O4-O4-O4; MFCD12546632; STL356002; 99%; AC-24630; As-69273; EPPS, Bioxtra,> 99.5% (Ukuthathwa); I-LS-113260; CS-0015183; FT-0616538; I-H0576; I-acid; n-2-hydroxyyethylpine-n-3-propasusulfonic acid; q1563525; w-10793525; I-W-107976; i-acid; (Hepps);
Impahla yamakhemikhali ye-Hepps
● Ukubukeka / Umbala: I-White Crystalline Powder
● Iphuzu elincibilikayo: 237-239 ° C (Lit.)
● Inkomba yokuqapha: 1.537
● I-PKA: 8.0 (ngo-25 ℃)
● Psa:89.46000
● Hlensity: 1.285 g / cm3
● logp: -0.16920
● Isitoreji se-temp.:store ku-rt.
● I-Solubity.Ke: 1 m ku-20 ° C, icacile, ingenamibala
● I-SOLUBLICISITILY.IBHODI ELINGCWELE. 25.2 g / l (20 ° C)
● xLogpp3: -3.6
● I-Hydrogen Bond Donor Count: 2
● Isibalo sakwamandla e-hydrogen
● I-Rotatable Bond Count: 6
● Isisindo esiqondile: 252.11437830
● Isibalo se-athomu esindayo: 16
● Ukuba yinkimbinkimbi: 280
Imininingwane Ephelile
● pictograph (s):
Xi
● Amakhodi Ayingozi: XI
● Izitatimende: 36/37/38-22
● Izitatimende Zokuphepha: 24/15-36-26
-Sizayo
Amakilasi Amakhemikhali:Ama-nitrogen compounds -> Amapayipi
Ukumamatheka kwe-canonical:I-C1CN (CCN1CCCS (= o) (= o) o) CCO
Sebenzisa:I-EPPS iyi-buffer evame ukusetshenziswa kahle. Imvamisa isetshenziswa njengehlukanisi e-Ultrathin isoelectric igxile egxile kwama-gels futhi ithuthukise ukulungiswa kwe-phosphoglucomutase. Isetshenziswa njenge-ejenti ebuhlungu ku-biology kanye ne-biochemistry.
Isingeniso Esinemininingwane
I-EPPS imi ye-N- (2-HydroxYethyl) Piperazine-N '- (3-Propasesulfonic Acid). Ungumenzeli we-zwitterionic buffering ageffering evame ukusetshenziswa ku-biological and biochemical cwaninga.- 10 amaphuzu athile asemqoka mayelana ne-EPPS:
Izakhiwo Ezihlabelayo:I-EPPS iyi-ejenti ephumelelayo ebuhlungu esiza ukugcina i-PH eqhubekayo yokusetha kokuhlola. Inenani le-PKA le-8.09, elenza lilungele ukugcwala kuhlu lwe-PH lwe-7.0-9.0.
Ukuqina:I-EPPS ikhombisa ukuqina okuhle kwamazinga okushisa ahlukahlukene futhi kuthinteka kancane izinguquko zokushisa ngokuqhathaniswa namanye ama-ejekeli abubi afana nama-tris nama-phosphate buffers. Lokhu kuzinza kwenza kufanelekile ukusetshenziswa kuzivivinyo ze-biochemical nezemvelo ezidinga izimo ezilawulwayo ze-PH.
Izicelo zebhayoloji:I-EPPS isetshenziswa kakhulu ezifundweni ezahlukahlukene zebhayoloji, njengokuhlanzwa kwamaprotheni, amehlo enzyme, kanye ne-DNA / RNA ISITOLO. Umthamo wayo wokubuphazamisa nokuqina ku-Ph PH PH kwenza kube yigugu kulezi zinhlelo zokusebenza.
Isiko Leselula:I-EPPS ingasetshenziswa futhi njenge-ejenti ebuhlungu emithonjeni yeMedia Yeseli. Kuyasiza ukugcina imvelo ezinzile ye-PH iyakwazi ukukhula nokunakekelwa kwezinhlobo ezithile zeseli.
Ion kanye ukuhambisana:I-EPPS iyindawo ye-zwitterionicic, okusho ukuthi iqukethe amacala alungile futhi abi. Lesi sici siyivumela ukuthi ihlawule ngempumelelo i-acidic ne-ayisisekelo. Ihambisana nama-molecule ahlukahlukene okuzalwa futhi yaziwa ukuthi ingenele okuncane okuphazanyiswa kwe-enzymatic.
Lapho usebenzisa ama-EPPs, kubalulekile ukulandela imihlahlandlela enconyiwe yokuhlushwa kanye nemihlahlandlela enikezwe ngumenzi. Ngokwengeziwe, hlinzeka ngaso sonke isikhathi futhi ulandele amaphrothokholi afanele ezokuphepha lapho uphatha noma yiliphi umenzeli wamakhemikhali noma we-buffering.
Isicelo
I-Hepps (N- (2-HydroxyEthyl) Piperazine-N'-3-Propasesulfonic) i-zwitterionic buffer evame ukusetshenziswa ocwaningweni lwemvelo nolwenziwe nge-biochemical. Ezinye izinhlelo zayo zifaka:
Isiko Leselula: IHepps isetshenziswa njenge-ejenti ebuhlungu emithonjeni yabezimo yamaseli ukuze ilondoloze i-PH eqhubekayo futhi inikeze indawo efanelekile yokukhula kwamaseli nokwanda. Kuyasiza ukuqinisa i-PH ngaphakathi kwebanga lomzimba futhi kugweme noma imiphi imiphumela emibi ezinqubweni zamaselula.
I-Enzyme Abs:I-Hepps ivame ukusetshenziswa njenge-buffer in enzyme asebetha ukwenza ngcono izimo ze-PH ezidingekayo ukuze uthole umsebenzi omkhulu we-enzyme. Umthamo wayo wokubuphazamisa usiza ukugcina i-ph ezinzile, eqinisekisa ukulinganisa okunembile ne-enzyme ethembekile.
Ukuhlanzwa kwamaphrotheni:IHepps ingasetshenziswa njenge-buffer ezinhlokweni zokuhlanza ama-protein. Kuyasiza ukugcina ukuqina kanye nomsebenzi wamaprotheni ngesikhathi sokuhlanzwa kwezinyathelo, njengekholomu i-chromatography noma ukuhlanzwa kwamaprotheni kusetshenziswa amathegi okuhambisana.
I-electrophoresis:I-Hepps kwesinye isikhathi isetshenziswa njenge-buffer e-gel electrophoresis yokwahlukana nokuhlaziywa kwamaprotheni nama-nucleic acid. Umthamo wayo we-PH Buffering usiza ukugcina i-ph eqinile ngesikhathi sokugijima kwe-electrophoretic, ukusiza ekuhlukaniseni okunembile kwama-molecule.
Amasu we-Molecular Biology:IHepps ingasetshenziswa njenge-buffer kumasu ahlukahlukene we-molecular biologres, kufaka phakathi i-PCR (Polymerase Chain Reaction), ukulandelana kwe-DNA, kanye nokuphendula kwe-enzymatic. Amandla alo obuhlungu aqinisekisa izimo ze-PH ezisebenzayo zokuphendula okuphumelelayo nangokufanele.
Izifundo ezibandakanya amaprotheni kalwelwesi:I-Hepps ilungele kahle ukufunda amaprotheni okuthuthuka ngenxa yezakhiwo zayo ezingena ezinhlamvbrane. Imvamisa isetshenziswa njenge-buffer ezivivinyweni ezibandakanya ukucaciswa okusebenzayo noma ukubuyiselwa kabusha kwamaprotheni okulima.
Kubalulekile ukuqaphela ukuthi ukugxila okuphelele kanye ne-PH ye-Hepps kungahluka ngokuya ngohlelo oluthile noma izimo zokuhlola. Ngakho-ke, kungcono ukuthintana nezincomo zezincwadi noma iziphakamiso zabahlinzeki ngokusetshenziswa okufanele kanye nokuhlushwa kwe-hepps ekuhlolweni kwakho.
