Imikhiqizo
HEPES usawoti we-sodium
Omqondofana:HEPES sodium usawoti;75277-39-3;HEPES hemisodium usawoti;103404-87-1;Sodium 2-(4-(2-hydroxyethyl)piperazin-1-yl)ethanesulfonate;sodium 2-[4-(2-hydroxyethyl) )piperazin-1-yl]ethanesulfonate;HEPES (sodium);C8H17N2NaO4S;4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid hemisodium salt;1-Piperazineethanesulfonic acid, 4-(2-hydroxyethyl)-, monosodium salt;4-(2-Hydroxyethyl)piperazine-1- i-ethanesulfonic acid usawoti we-sodium; 4-(2-hydroxyethyl)piperazin-1-ylethanesulphonate;UNII-Z9FTO91O8A;Z9FTO91O8A;sodium;2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonate;DTXSID4044458;CHEBI:4675;CHEBI:4675; 278-169-7;1-Piperazineethanesulfonic acid, 4-(2-hydroxyethyl)-, sodium salt (1:1);N-(2-Hydroxyethyl)piperazine-N'-2-ethanesulfonic acid,sodium salt;HEPES sodium usawoti, > = 99.5% (titration); i-sodium i-hepes;N-(2-Hydroxyethyl)piperazine-N'-(2-ethanesulfonic acid) usawoti wesodium;MFCD00036463;HEPES, usawoti wesodium;HEPES usawoti wesodium, 98%;C8H18N2O4S.Na;SCHEMBL229142;WAS-14;CHEMBL3187284;DTXCID2024458;RDZTWEVXRGYCFV-UHFFFAOYSA-M;C8-H18-N2-OPE-S usawoti we-sodium; (1M);Tox21_302155;HB5187;N-(2-Hydroxyethyl)piperazine-N-(2-ethanesulfonicacid)hemisodiumsalt;HEPES sodium usawoti, biochemical ibanga;AKOS015897769;AKOS015912229;AKOS015964204;NCGC00255791-01;CAS-75277-39-3;HY-108535;CS-0029103;FT-061030863;6F6; N'-hydroxyethyl-N-piperazineethanesulfonate;HEPES sodium usawoti, BioXtra, >=99.0% (titration);HEPES sodium sodium, Vetec(TM) reagent grade, 96%;W-104397;Q27120698;Sodium2-(4-(2-hydroxyethyl)piperazin-1-yl)ethanesulfonate;4-(2-Hydroxyethyl) -1-piperazineethanesulfonic acid sodium salt;4-(2-hydroxyethyl)- 1-piperazineethanesulfonic acid, monosodium usawoti;N-(2-Hydroxyethyl)piperazine-N'-(2-ethanesulfonic acid)sodiumsalt;N-2-Hydroxyethylpiperazine-N'-2-ethanesulphonic acid hemisodium usawoti;HEPES sodium usawoti, BioPerformance Certified, ilungele isiko lamaseli, =99.5%
I-Chemical Property ye-HEPES Sodium Salt
● Ukubukeka/Umbala:impushana emhlophe
● I-Vapor Pressure:0Pa ku-25℃
● Indawo Encibilika:234 °C
● PKA:7.5(at 25℃)
● I-PSA:92.29000
● Ukuminyana:1.504[ku-20℃]
● I-LogP:-0.90190
● Ukushisa Kwesitoreji: Gcina e-RT.
● Kuyazwela.:Hygroscopic
● Ukunyibilika.:H2O: 1 M ku-20 °C, kucacile, akunambala
● Ukunyibilika kwamanzi.:Iyancibilika emanzini.
● Isibalo Somnikeli Webhondi Ye-Hydrogen:1
● Isibalo Sokwamukela Ibhondi Ye-Hydrogen:6
● Isibalo Sebhondi Eshintshayo:5
● IMisa Langempela:260.08067248
● Isibalo se-athomu esindayo:16
● Ukuba yinkimbinkimbi:272
Ulwazi Oluphephile
● Ama-Pictogram:
● Amakhodi Wengozi:
● Izitatimende Zokuphepha:22-24/25
Iwusizo
Amakilasi Amakhemikhali:Okunye Ukusetshenziswa -> Izibhafa Zebhayoloji
I-Canonical SMILES:C1CN(CCN1CCO)CCS(=O)(=O)[O-].[Na+]
Isebenzisa:Isixazululo se-HEPES sikasawoti we-sodium silungele izifundo zesiko lamaseli. Ingasetshenziswa ekulungiseni isisombululo se-buffer se-HEPES. Ingasetshenziswa njenge-buffer ngesikhathi sokuhlolwa kwe-phosphorylation kuma-fibroblasts e-permeabilized. Ingase isetshenziselwe ukuhlanganisa i-dialysis buffer eyinkimbinkimbi, esetshenziswe ekunqumeni i-thyroxin yamahhala ku-serum nge-radioimmunoassay. Isixazululo se-HEPES sikasawoti we-sodium silungele ukusetshenziswa ezifundweni zesiko lamaseli. Ingasetshenziswa ekulungiseni isisombululo se-buffer se-HEPES. Ingasetshenziswa njenge-buffer ngesikhathi sokuhlolwa kwe-phosphorylation kuma-fibroblasts e-permeabilized. Ingase isetshenziselwe ukuhlanganisa i-dialysis buffer eyinkimbinkimbi, esetshenziswe ekunqumeni i-thyroxin yamahhala ku-serum nge-radioimmunoassay.
Isingeniso Esiningiliziwe
HEPES usawoti we-sodium, eyaziwa nangokuthi i-4-(2-hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt, iwuhlobo olusetshenziswa kakhulu lwe-HEPES. Kuyinhlanganisela ye-zwitterionic ephilayo esebenza njenge-ejenti ephazamisayo ocwaningweni lwebhayoloji nolwe-biochemical.
Usawoti we-sodium we-HEPES uvame ukusetshenziswa kumidiya ye-cell culture kanye namabhafa ebhayoloji ngenxa yekhono lawo lokugcina i-pH ezinzile ezungeze izimo zomzimba (pH 7.2 - 7.6). Incibilika kakhulu emanzini futhi inobuthi obuphansi uma kuqhathaniswa, okwenza ifanelekele ukusetshenziswa okuhlukahlukene.
Ifomu le-sodium likasawoti we-HEPES likhethwa ezinhlelweni eziningi njengoba lithuthukisa ukuncibilika nokuzinza, uma kuqhathaniswa nefomu le-asidi yamahhala. Itholakala ngezohwebo njengempushana emhlophe ecwebezelayo engancibilika kalula emanzini ukuze kulungiswe izixazululo zokusebenza.
Abacwaningi bavame ukusebenzisa usawoti we-HEPES wesodium kumasu ebhayoloji yamangqamuzana, isiko lamaseli, ukuhlola ama-enzyme, ukuhlanzwa kwamaprotheni, nokunye ukuhlola kwamakhemikhali ezinto eziphilayo lapho ukulawula i-pH kubalulekile. Umthamo wayo wokubhafa nokuhambisana nezinhlelo zebhayoloji kuyenza ibe ukukhetha okudumile ekugcineni izimo ezilungile zomsebenzi nokuzinza kwama-molecule ahlukahlukene ebhayoloji.
Ngaphambi kokusebenzisa i-HEPES kasawoti wesodium kunoma isiphi isicelo, kubalulekile ukubuyekeza izincwadi, uthintane nezincomo zabahlinzeki, futhi wandise ukugxila kanye nobubanzi be-pH ngokusekelwe ezidingweni ezithile zokuhlola.
Isicelo
Usawoti we-sodium we-HEPES uthola ukusetshenziswa okubanzi ezindaweni ezahlukahlukene zocwaningo lwebhayoloji kanye ne-biochemical. Ezinye zezinsiza zayo ezibalulekile zihlanganisa:
I-Cell Culture:Usawoti we-sodium we-HEPES uvamise ukungezwa kumithombo yezokuxhumana yamaseli ukuze kugcinwe i-pH ezinzile phakathi kwebanga le-physiological futhi unikeze indawo efanelekile ukuze amaseli akhule futhi ande.
Umenzeli wokulondoloza imali:Usawoti wesodium we-HEPES uvame ukusetshenziswa njenge-ejenti ephazamisayo kumabhafa nezixazululo zebhayoloji, okuhlanganisa ukuhlolwa kwama-enzyme, ukuhlanzwa kwamaprotheni, nezindlela zebhayoloji yamangqamuzana. Isiza ukugcina i-pH engaguquki ngokumelana nezinguquko ku-acidity noma i-alkalinity.
I-Electrophoresis: Usawoti we-sodium we-HEPES usetshenziswa njengesivimbeli ku-gel electrophoresis ukuze kugcinwe i-pH ezinzile ngesikhathi sokuhlukaniswa kwama-nucleic acid noma amaprotheni. Ivimbela ukushintsha kwe-pH okungase kuthinte amaphethini okufuduka nokuhlukaniswa kwama-molecule kujeli.
Amaprotheni Ukuzinza: Usawoti we-sodium we-HEPES wengezwa ezixazululweni zamaphrotheni ukuze uzinzise ukwakheka kwazo nokugcina umsebenzi wazo. Isiza ukugcina imvelo ye-pH efanelekile edingekayo ukuze kuqiniswe amaprotheni nokusebenza.
Umsebenzi we-Enzyme: Usawoti we-sodium we-HEPES usetshenziswa njengesivimbeli ekuphenduleni kwe-enzymatic ukuze kugcinwe i-pH edingekayo ukuze uthole umsebenzi ophelele we-enzyme. Kuyasiza ukuqinisekisa ukuthi ama-enzyme asebenza ngokusebenza kwawo okuphezulu nokunemba.
I-Live Cell Imaging:Usawoti we-sodium we-HEPES uvame ukufakwa emithonjeni yezithombe ukuze kuhlolwe bukhoma izithombe zamaseli. Umthamo wayo wokubhafa usiza ekugcineni i-pH oyifunayo futhi ivimbele ukushintshashintsha okungase kuthinte ukusebenza kanye ne-fluorescence yamaseli.
Amasu e-Molecular Biology: Usawoti we-sodium we-HEPES usetshenziswa kabanzi kumasu ahlukahlukene ebhayoloji yamangqamuzana, okuhlanganisa i-DNA noma i-RNA isolation, i-PCR, ukulandelana kwe-DNA, nokuhlaziywa kwamaprotheni. Amandla ayo okubhafa avumela imiphumela enembile nethembekile phakathi nalezi zinqubo.
Kubalulekile ukuqaphela ukuthi ukusetshenziswa okukhethekile kanye nokugxiliswa kukasawoti wesodium ye-HEPES kungase kwehluke kuye ngezidingo zokuhlolwa kanye nesistimu yebhayoloji esicwaningwayo. Ngakho-ke, kuyatuseka ukuthintana nezincwadi nezincomo zabahlinzeki ukuze zisetshenziswe ngokugcwele.
