Imikhiqizo
IHepes sodium usawoti; Case No.: 75277-39
Amanyanjana: Hepes sodium usawoti; 75277-39; Hepes Hemisodium usawoti; 103404-87-1; (4-hydroxyethyl) poper (sodium); c8h17n2Nao4s; usawoti; sodium 4- (2-hydroxyyethyl) piperazin-1-ylethanesphonate; Unii-z9fto91a8a; z9fto91o8a; i-sodium; 2- [4- (2-hydroxyyethyl) Piperesazin-1-yl] ethanesulfonate; dtxsid4044458; Chebi: 46758; Ama-EINEC 278-169-7; 1-Piperazineethanusulfonican, 4- (2-hydroxyethyl) -, usawoti we-sodium (1: 1); N- IHepes; I-98%; c8h18n2o4s.A; Stchem 229142; wayengu-14; i-chembcid20NOOYCHFV; (1m); I-TOX21_302155; HB5187; N- (2-HydroxYethyl) Piperazine-n- (2 ibanga; Akos015897769; Akos015912229; N'-hydroxyswethyl-n-pipererazineethanes I-96%; I-W-104397; Q27120698; I-Sodium2- (4-yl) ethanesulfonate; i-monosodium usawoti;
Impahla yamakhemikhali ye-hepes sodium usawoti
● Ukubukeka / umbala: i-powder emhlophe
● Ingcindezi ye-Vapor: 0Pa ngo-25 ℃
● Iphuzu elincibilikayo: 234 ° C
● I-PKA: 7.5 (ngo-25 ℃)
● Psa:92.29000
● Ukuqina: 1.504 [At 20 ℃]
● Logp: -0.90190
● Isitoreji se-temp.:store ku-rt.
● I-SENTITE.:HGroscopic
● I-Solubity.Ke: 1 m ku-20 ° C, icacile, ingenamibala
● I-SOLUBLITILIZO YAMANZI.:
● I-Hydrogen Bond Donor Count: 1
● Isibalo sakwamandla e-hydrogen
● I-Rotatable Bond Count: 5
● Isisindo esiqondile: 260.08067248
● Isibalo se-athomu esindayo: 16
● Ukuba yinkimbinkimbi: 272
Imininingwane Ephelile
● pictograph (s):
● Amakhodi Avikelekayo:
● Izitatimende zokuphepha: 22-24 / 25
-Sizayo
Amakilasi Amakhemikhali:Okunye ukusetshenziswa -> ama-buffers e-biological
Ukumamatheka kwe-canonical:I-C1CN (CCN1CCO) CCS (= o) (= o) [O -]. [NA +]
Sebenzisa:Isixazululo sikaSawoti se-Hepes Sodium silungele izifundo zesiko leselula. Ingasetshenziswa lapho kulungiswa isisombululo se-buffer se-hefes. Ingahle isetshenziswe njenge-buffer ngesikhathi se-phosphorylation atholwa ku-fibroblasts evunyelwe. Ingasetshenziswa ukuqamba i-dialysis buffer eyinkimbinkimbi, isetshenziswe ekunqumeni kwamahhala i-thyroxin ku-serum yi-radiommunoas. Isixazululo sikaSawoti we-Hepes Sodium silungele ukusetshenziswa ezifundweni zama-Cell Culture. Ingasetshenziswa lapho kulungiswa isisombululo se-buffer se-hefes. Ingasetshenziswa njenge-buffer ngesikhathi se-phosphorylation atholwa ku-FibroBlasts evunyelwe. Ingasetshenziswa ukuqamba i-dialysis buffer eyinkimbinkimbi, isetshenziswe ekunqumeni kwamahhala i-thyroxin ku-serum yi-radiommunoas.
Isingeniso Esinemininingwane
Usawoti we-sodium we-hepes, eyaziwa nangokuthi ngu-4- (2-hydroxyethyl) i-piperazine - 1-ethanusulfonicid sodium usawoti, iyindlela esetshenziswa kakhulu ye-hepes. Kuyindawo ye-zwitterionic organic organic esebenza njenge-ejenti ebukhali ekucwaningeni kwemvelo kanye ne-biochemical.
Usawoti we-hepes sodium uvame ukusetshenziswa emithonjeni yezempi yamaseli kanye nama-biological buffers ngenxa yekhono lawo lokugcina ibanga elizinzile PH azungeze izimo zomzimba (PH 7.2 - 7.6). Kuyancibilika kakhulu emanzini futhi inenobuthi obuphansi, okwenza kube lulungele izinhlelo zokusebenza ezahlukahlukene.
Ifomu likasawoti le-sodium le-hepes likhethwa kuzinhlelo zokusebenza eziningi njengoba lithuthukisa i-solubity nokuqina, ngokuqhathaniswa nefomu lamahhala le-acid. Itholakala ngokuhweba njenge-white crystalline powder engachithwa kalula emanzini ukulungiselela izixazululo zokusebenza.
Abaphenyi bavame ukusebenzisa usawoti we-hedes sodium kumasu we-molecular biologres, amasiko e-cell, absousa Umthamo wayo wokuhlanza kanye nokuhambisana nezinhlelo zemvelo kwenza kube yikhetho elithandwayo lokugcina izimo ezifanele zomsebenzi nokuqina kwama-molecule ahlukahlukene okuzalwa.
Ngaphambi kokusebenzisa usawoti we-hepes sodium kunoma yiluphi uhlelo lokusebenza oluthile, kubalulekile ukuze ubukeze izincwadi ezithile, bhekana nezincomo zabahlinzeki, futhi uthuthukise ububanzi bokuhlushwa nobunye ngokususelwa kwizidingo ezithile zokuhlola.
Isicelo
IHepes Sodium usawoti ithola isicelo esisakazekile ezindaweni ezahlukahlukene zokucwaninga ngebhayoloji kanye ne-biochemical. Okunye ukusetshenziswa kwalo kokhiye kufaka phakathi:
Isiko Leselula:Usawoti we-hepes sodium uvame ukungezwa kumithombo yezinsizakalo zesiko leselula ukuze alondoloze i-PH ezinzile ngaphakathi kwebanga lomzimba futhi anikeze indawo efanelekile yamaseli ukuze ikhule futhi ikwazi ukwanda.
Umenzeli we-Buffering:Usawoti we-hepes sodium uvame ukuqashwa njenge-ejenti ebukhali ezitolo ezinkulu zemvelo kanye nezixazululo, kufaka phakathi izihlakala ze-enzyme, ukuhlanzwa kwamaprotheni, kanye namasu we-molecular biology. Kuyasiza ukugcina njalo ph ngokumelana nezinguquko ku-acidity noma i-alkalinity.
I-electrophoresis: Usawoti we-hepes sodium usetshenziswa njenge-buffer e-Gel Electrophoresure ukuze alondoloze i-ph eqinile ngesikhathi sokuhlukaniswa kwama-nucleic acid noma amaprotheni. Kuvimbela amashifu ku-PH athinta ukufuduka kanye namaphethini wokuhlukanisa ama-molecule ejeli.
Ukuqina kwamaphrotheni: I-Hepes sodium usawoti wengezwa kwizixazululo zamaprotheni ukuze aqinise ukwakheka kwawo futhi agcine umsebenzi wawo. Kuyasiza ukugcina indawo efanelekile ye-PH edingekayo ukuze uzinqumo kwamaprotheni nokusebenza.
Umsebenzi we-enzyme: Usawoti we-hepes sodium usetshenziswa njenge-buffer ekuphendukeni kwe-enzymatic ukugcina i-ph efiselekayo yomsebenzi ofanele we-enzyme. Kuyasiza ekuqinisekiseni ukuthi umsebenzi we-enzyme ngokusebenza kwawo okuphezulu nokunemba.
Ukulingisa kweseli bukhoma:Usawoti we-hepes sodium uvame ukufakwa kwimithombo yezindaba ecabangayo yokuhlolwa kwezibuko ezibukhoma ezifanekisweni. Umthamo wayo wokuhlanza isiza ukugcina i-PH oyifunayo futhi ivimbele ukuguquguquka okungathinta ukusebenza kwe-viality kanye ne-fluorescence of the cell.
Amasu we-Molecular Biology: Usawoti weSodium we-Hepes usebenza kabanzi kumasu ahlukahlukene we-molecular biologres, kufaka phakathi i-DNA noma i-RNA yodwa, i-PCR, i-DNA Ukulandelana, kanye nokuhlaziywa kwamaprotheni. Amandla alo abubi avumela imiphumela enembile nethembekile ngalezi zinqubo.
Kubalulekile ukuqaphela ukuthi uhlelo lokusebenza oluthile kanye nokuhlushwa kwe-hepes sodium usawoti kungahluka ngokuya ngezidingo zokuhlola kanye nohlelo lwebhayoloji olufundwayo. Ngakho-ke, kungakuhle ukubonana nezincomo nezincwadi zabahlinzeki ukuze kusetshenziswe kahle.
