Imikhiqizo
Hepes; Case No.:7365-45-9
Amanyanjana: 1- [2-hydroxyethyl) -1-piperazinyl] ethane-2-sulfonicinicacid; 2- [4-hydroxyethyl) piperaziny] I-ethanulfonic acid; 4- (2-hydroxyethyl) piperazine-1- (2-ethanusulfonic i-acid); i-acid); N'-2-hydroxyyethylpine-n-2-ethanusulfonic acid; tvz 7; bebeneminyaka engu-13;
Impahla yamakhemikhali yeHepes
● Ukubukeka / Umbala: I-White Crystalline Powder
● Ingcindezi ye-Vapor: 0Pa ngo-25 ℃
● Iphuzu elincibilikayo: 234-238 ° C
● Inkomba ebonakalayo: N20 / D 1.339
● Iphuzu elibilayo: 408 ℃ [ngo-101 325 pa]
● I-PKA: 7.5 (ngo-25 ℃)
● Psa:89.46000
● Hlensity: 1.325 g / cm3
● Logp: -0.55930
● Isitoreji Temp.:2-8
● I-Solubity.Ke: 1 m ku-20 ° C, icacile, ingenamibala
● Amanzi Solubilicity.:SOLUble
● xLogpp3: -4.1
● I-Hydrogen Bond Donor Count: 2
● Isibalo sakwamandla e-hydrogen
● Ukulondolozwa kwebhondi okujikelezayo: 4
● Isisindo esiqondile: 238.09872823
● Isibalo se-athomu esindayo: 15
● Ukuba yinkimbinkimbi: 254
Imininingwane Ephelile
● pictograph (s):
Xi
● Amakhodi Ayingozi: XI
● Izitatimende: 36/37/38
● Izitatimende Zokuphepha: 24/122-36-26
-Sizayo
Ukumamatheka kwe-canonical:I-C1CN (CC [NH +] 1CCS (= o) (= o) [O -] CCO
Incazelo:IHepes ichazwe njengenye yezinhloso ezinhle kakhulu zezinhloso zonke ezitholakalayo ngocwaningo lwebhayoloji. E-biological pH, i-molecule yi-zwitterionicic, futhi iyasebenza njenge-buffer kwaPh 6.8 kuya ku-8.2 (PKA 7.55). Isetshenziswa ngokujwayelekile kusiko leseli ekuhlungeleni phakathi kuka-5mm kuya ku-30 mm. IHepes isetshenziswe ezinhlobonhlobo zezicelo, kufaka phakathi isiko lezicubu. Kuvame ukusetshenziswa kwabezindaba zamaseli we-buffer emoyeni. IHepes ithola ukusetshenziswa kwayo ekuhlolweni kwe-vitro ku-MG.
Sebenzisa:Okungelona ubuthi esitokisini. Isetshenziswa njenge-hydrogen ion buffer, engalawula uhla lwe-PH yesikhathi eside. Ukuhlushwa kungu-10-50mmol / l. Ngokuvamile, i-20mmol / i-LHEPS kwikhambi lezakhi zingathola ikhono le-buffer. I-Biological Buffer. IHepes iyi-buffer ejwayelekile yesayensi yemvelo, esetshenziswa ikakhulukazi kumasiko weseli ukugcina i-pH PH PH. Isebenza ngengxenye yokububula, esetshenziswa lapho kulungiswa ama-buffers. Kuchazwa njengenye yezinto ezihle kakhulu zezinhloso zenhloso zonke ezitholakalayo ecwaningayo eziphilayo.Kusetshenziswe njengengxenye yesawoti elilinganiselayo, esetshenziselwa ukulungiswa kwezilinganiso zezicubu zomzimba, ezisetshenziselwa ukulungiswa kwezicubu ze-basolatal esihlahleni lwe-bacholateral of amasiko.
Isingeniso Esinemininingwane
I-HepesImele 2- (4- (2-hydroxyethyl) pipersulfonic-1-yl) ethanusulfonic acid. Kuyi-ejenti elihlangene elivame ukusetshenziswa ocwaningweni lwemvelo nolwenziwe nge-biochemical. IHepes isiza ukugcina i-PH ezinzile kwabezindaba zamaseli, njengoba ikwazi ukwamukela nokunikela ama-protons, ngaleyo ndlela kulawule izinguquko ze-PH. Isakhiwo saso samakhemikhali nezakhiwo zikwenza kusebenze kakhulu ekugcineni i-pH eqhubekayo futhi efanelekile yokuphendula okuningi kwezinto eziphilayo kanye ne-enzymatic. IHepes isetshenziswa kakhulu kumasiko weseli, ukuhlanzwa kwamaprotheni, kanye namasu we-molecular biology.
Isicelo
IHepes inezicelo eziningi emasimini ahlukahlukene ocwaningo. Ezinye zezicelo ezijwayelekile zeHepes yilezi:
Isiko Leselula: IHepes isetshenziswa kakhulu njengengxenye kwabezindaba zesiko leselula ukuze balondoloze imvelo ye-PH ezinzile. Kuyasiza ulawuse izinguquko ze-PH ezibangelwa yi-Metabolic imisebenzi kanye nokuqongelelwa kwemikhiqizo yemfucuza kumasiko weseli.
I-Enzyme Abs:IHepes ivame ukusetshenziswa njenge-ejenti ebukhali ezinhlobonhlobo ze-enzyme ukuze ilondoloze i-pH eqhubekayo, okubaluleke kakhulu ekusebenzeni kahle kwama-enzyme. Kuyasiza ekuqinisekiseni imiphumela efanelekile nenempumelelo.
I-electrophoresis:I-HEPES isetshenziswa njenge-ejenti ebukhali kumasu we-electrophoresis ake polyracrylamide gel electophoresis (ikhasi) kanye ne-agarose gel electrophoresophoresoresis. Kuyasiza ukugcina uhla olufunayo lwe-PH lokuhlukaniswa kahle nokuhlaziywa kwama-macromolecule afana namaprotheni nama-nucleic acid.
Ukuhlanzwa kwamaphrotheni:IHepes kwesinye isikhathi ifakiwe kuma-protein anpitification buffers ukuze alondoloze i-ph oyifunayo ngezinyathelo ezihlukile zenqubo yokuhlanzwa. Kuyasiza ukugcina ukuqina kanye nomsebenzi wamaprotheni ngesikhathi sezinyathelo zokuhlanzwa.
Amasu we-Molecular Biology:IHepes ingasetshenziswa kumasu ahlukahlukene we-molecular biologres, njenge-PCR (Polymerase Chain Reaction) kanye nokulandelana kwe-DNA, ukugcina i-pH eqinile. Kuyasiza ukwenziwa ngcono ukusebenza kwama-enzyme futhi kuqinisekise imiphumela enembile nethembekile.
Kubalulekile ukuqaphela ukuthi ukugcwaliseka okuthile kwe-hepes kanye nesicelo sawo kungahluka ngokuya ngezimo zokuhlola nezidingo.
