Imikhiqizo
3-MorpholinoproPopasesulfonican acid; Case No.: 1132-61-2
Amanyanjana: 3-morpholin-4-ium-4-sulfopane-1-sulfonate; Chebi: 39076;
Impahla yamakhemikhali ye-3-Morpholinopropropasesulfonic acid
● Ukubukeka / umbala: i-powder emhlophe
● Ingcindezi ye-Vapor: 0Pa ngo-25 ℃
● Iphuzu elincibilikayo: 277-282 ºC
● Inkomba evulekayo: 1.512
● I-PKA: 7.2 (ngo-25 ℃)
● I-Flash Point: 116 ºC
● Psa:75.22000
● Hlensity: 1.298 g / cm3
● Logp: 0.61520
● Isitoreji se-temp.:store ku-rt.
● I-Solubity.:HO: 1 m ku-20 ° C, kucacile
● Amanzi e-solubility.:1000 g / l (20 ºc)
● xLogp3: -3.2
● I-Hydrogen Bond Donor Count: 1
● Isibalo sakwama-Hydrogen Bond samukelwa: 4
● I-rotatable bond count: 3
● Isisindo esiqondile: 209.07217913
● Isibalo se-athomu esindayo: 13
● Ukuxaka: 214
Imininingwane Ephelile
● pictograph (s):
Xi
● Amakhodi Ayingozi: XI
● Izitatimende: 36/37/38
● Izitatimende zokuphepha: 26-36
-Sizayo
Ukumamatheka kwe-canonical:C1cocc [nh +] 1cccs (= o) (= o) [o-]
Incazelo:I-MOPS (3-Morpholinopropasesulfonican) i-buffer eyethulwa yi-Good et al. Ngawo-1960s. Kuyinto analog ehlelekile kuma-mes. Isakhiwo saso samakhemikhali siqukethe indandatho ye-morpholine. IHepes iyisizinda esifanayo se-PH SHFFING esiqukethe indandatho yePiperazine. Nge-PKA ka-7.20, ama-mops angu-buffer omuhle kakhulu wezinhlelo eziningi zemvelo eziseduze ne-Ph.Tral Ph.It isetshenziswa njenge-ejenti yokwenziwa ye-agent engezansi kwe-PH 7.5.
Sebenzisa:3- (n-Morpholino) I-PropasesulfonicFonic Acid noma ama-mops ngenxa yemvelo yayo ye-inert iyinhlangano ekhethwayo esetshenziswayo ezifundweni eziningi ze-biochemical eziphakathi nendawo enikeziwe I-dilting fungal inoculum.as i-buffer e-capillary-zone electrophoresis ukuze ihlolwe ukusebenza kahle. I-MOPS isebenza njengomenzeli onobumba obuningi obusetshenziswa ocwaningweni oluhlukahlukene lwebhayoloji. I-MOPS isetshenzisiwe njenge: Ingxenye yesiko le-cell cellent eLentiviral Particle ProductAs i-ejenti evuthayo ekukhuleni okuphakathi kwe-microbial kanye ne-nuclei.
Isingeniso Esinemininingwane
I-3-Morpholinopropropasesulfonic acid, eyaziwa nangokuthi ama-mops, yiyonamba yamakhemikhali evame ukusetshenziswa njenge-ejenti ebuhlungu ezisekelweni ezahlukahlukene zebhayoloji kanye ne-biochemical. Kuyimpuphu we-crystalline emhlophe enyakazisa emanzini.
I-MOPS inefomula yamakhemikhali ye-C7H15NO4S kanye nesisindo samangqamuzana esingu-209.26 g / mol. Inenani le-PKA elicishe libe ngu-7.2, okwenza kube yi-buffer ephumelelayo yokugcina i-phiph ezungeze i-7.0-7.5.
Okunye kokusetshenziswa okuyisisekelo kwama-mops ku-biology ye-molecular kanye nocwaningo lwe-biochemistry ukulawula i-PH kwezinto ezahlukahlukene zebhayoloji nasezihlotsheni. Isetshenziswa kakhulu kuzinhlelo zokusebenza ezinjenge-DNA / RNA Ukuhlukaniswa, ukukhishwa kwamaprotheni, ukusabela kwe-enzymatic, ne-gel electrophoresis. I-MOPS ihlinzeka ngokulawula okuzinzile futhi okungaguquguquki kwe-PH, okuvumela abaphenyi ukuthi balondoloze izimo ezinhle kakhulu zokuhlola kwabo.
Ama-mops nawo angasetshenziswa njengesixhumanisi kwimidiya yesiko leselula nakumasu we-Chromatography. Yaziwa nge-Ultraviolet yayo ephansi (UV) ebangeni lama-240-300 nm, okwenza lilungele ukusetshenziswa kuzinhlelo zokusebenza ezenzelwe i-UV.
Lapho usebenza nge-mops, kubalulekile ukuyiphatha ngokunakekelwa okufanele. Kufanele igcinwe endaweni epholile, eyomile futhi igcinwe kude nezinto ezingahambelani. Ukuphepha kokuphepha, njengokugqoka imishini efanelekile yokuvikela, kufanele kulandelwe lapho kuphathwa yile nhlanganisela.
Njenganoma imuphi amakhemikhali, kungakuhle ukubuyekeza ishidi ledatha yezokuphepha (SDS) bese uthintana nomenzi noma uchwepheshe ofanelekayo wokuphatha kanye nemiyalo yokusetshenziswa.
Isicelo
I-3-MorpholinoproPopasesulfonic acid (mops) inezicelo ezahlukahlukene emikhakheni ehlukene. Ezinye izinhlelo ezivamile zifaka:
Umenzeli we-Buffering:I-MOPS ivame ukusetshenziswa njenge-ejenti ebuhlungu ekuhlolweni kwebhayoloji kanye ne-biochemical. Kuyasiza ukugcina i-PH ezinzile, ikakhulukazi ebangeni lomzimba le-PH angu-7.0-7.5
I-Molecular Biology kanye ne-Biochemistry: I-MOPS isetshenziswa kabanzi njenge-buffer ku-DNA / RNA Ukuhlukaniswa, ukukhishwa kwamaprotheni, ukusabela kwe-enzymatic, ne-gel electrophoresis. Inikeza izimo ezinhle ze-PH zalezi zango futhi kuqinisekise imiphumela ethembekile.
Imidiya Yesiko Leseli: I-MOPS isetshenziswa njenge-ejenti yokuhlasela ekwakhekeni kwemidiya yeselula. Kuyasiza ukugcina i-PH ngaphakathi kohlu olufunayo, ukuhlinzeka indawo ezinzile yokukhula kwamaseli nokusebenza.
I-Chromatography:I-MOPS isetshenziswa njenge-buffer kumasu ahlukahlukene we-chromatography, kufaka phakathi i-ion Exchange Chromatography kanye ne-chromatography yosayizi yokukhishwa. Iqinisekisa izimo ze-PH ezingaguquki ngesikhathi sezinqubo zokuhlukanisa kanye nokuhlanza.
Synthesis yamakhemikhali: Ama-mops angaqashwa njenge-realent noma i-solvent ekuphendukeni kwamakhemikhali. Ukuqina kwayo, i-solubility, kanye nezakhiwo ze-PH buffering zenza zilungele izinhlelo ezahlukahlukene zokwenziwa.
UV spectroscopy:I-MOPS inokutholwa okuphansi ku-UV Range phakathi kuka-240-300 NM, okwenza kube lusizo ekuhlolweni kwe-UV spectroscopy. Ivumela ukukalwa okunembile ngaphandle kokuphazamiseka kusuka ekubumbeni uqobo.
Lezi yizibonelo ezimbalwa nje zokusetshenziswa okuningi kwe-3-Morpholinopropropasesulfonic acid (mops). Izakhiwo zayo eziguquguqukayo kanye nezindawo zokulawula i-PH zikwenza ithuluzi elibalulekile kumasethingi ahlukahlukene wesayensi nokucwaninga.
