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2- [Tris (hydroxymethyl) Methylamino] -1-ethanusulfonic acid; Case No.: 7365-44-8
![2- [Tris (HydroxyMethyl) Methylamino] -1-Ethanusulfonic acid 7365-44-8](http://www.pengnuochemical.com/uploads/2-Trishydroxymethylmethylamino-1-ethanesulfonic-acid-7365-44-8.png)
Amanyanjana: UChebi: 39036; 2 - [2-hydroxy-1,1-BIS (HydroxyMethyl) Ammonio} ethanoxylonate; n-tris (hydroxymethyl) methyl-2-ammonioneasesulfonate
Impahla yamakhemikhali ka-2- [Tris (HydroxyMethyl) Methylamino] -1-ethanusulfon acid
● Ukubukeka / Umbala: I-White Crystalline Powder
● Iphuzu elincibilikayo: ~ 223-225 ° C
● Inkomba evulekayo: 1.57
● I-PKA: 7.5 (ngo-25 ℃)
● PSA: 135.47000
● Hlensity: 1.554 g / cm3
● Logp: -1.34880
● Isitoreji Isitoreji. Isitolo se-RT.
● I-Solubity.Ke: 1 m ku-20 ° C, icacile, ingenamibala
● I-SOLUBLICILY YAMANZI.
● xLogp3: -5.8
● I-Hydrogen Bond Donor Count: 4
● Isibalo sakwamandla e-hydrogen
● I-Rotatable Bond Count: 6
● Isisindo esiqondile: 229.06200837
● Isibalo se-athomu esindayo: 14
● Ukuxaka: 220
Imininingwane Ephelile
● pictograph (s):
Xi
● Amakhodi Ayingozi: XI
● Izitatimende: 36/37/38
● Izitatimende zokuphepha: 22-24/15-36-26
-Sizayo
● Ukumamatheka kwe-canonical:C (CS (= O) (= o) [O -]) [NH2 +] c (co) (co) co
● Sebenzisa:I-analog ehlelekile ku-Tris Buffer. I-Biological Buffer. Ama-tes asetshenziswa i-ejenti esetshenziswa kakhulu.
Isingeniso Esinemininingwane
2- [Tris (HydroxyMethyl) Methylamino] -1-Ethanusulfon acid, eyaziwa nangokuthi ama-tes noma i-n-2-hydroxyyethylpine-n'-2-ethanusulfonincic, iyindawo yokuhlanza iZwitterionic
TesImvamisa iqashwa njenge-buffer ekuhlolweni kwelebhu ngenxa yekhono layo lokugcina imvelo eqinile ye-PH, ikakhulukazi ebangeni le-6.5 kuye ku-8.5. Iphinde ibe nokumunca ukukhanya okuphansi kokukhanya kwe-ultraviolet, okwenza kube lula ukusetshenziswa kuzinhlelo zokusebenza ze-spectrophotometric.Lokhu okuhlanganisiwe kungukuphazamiseka okuningi kwe-enzymatic, okuvumela izivivinyo ezithembekile.
TesIsetshenziswa kakhulu ezintweni eziphilayo kanye ne-biochemical amehlo, ukuhlanzwa kwamaprotheni, i-electrophoresis, kanye nemidiya yesiko leselula.
Kuyaqapheleka ukuthi ama-tes ane-hygroscopic, okusho ukuthi uthatha kalula umswakama evela emvelweni, ngakho-ke kubalulekile ukugcina nokuphatha kahle ukuze kugcinwe ukusebenza kahle.
Isicelo
2- [Tris (HydroxyMethyl) Methylamino] -1-Ethanesulfonic acid (tes) yindawo ewusizo eyaziwa ngokuyinhloko ngezakhiwo zayo ezibucayi. Okunye kokusetshenziswa kokhiye kwalo yile:
Umenzeli we-Buffering:Ama-tes asetshenziswa kakhulu njengengxenye yokuhlanza ekuhlolweni kwe-biochemical ne-molecular biology. Kuyasiza ukugcina imvelo ezinzile ye-PH, ikakhulukazi ebangeni le-6.5 kuye ku-8.5
AMAZWI EZIQINILE NOKUFUNDAAma-tes asetshenziswa ezindabeni ezahlukahlukene zebhayoloji kanye ne-biochemical lapho kugcinwa khona i-pH ethile yemiphumela efanelekile kubalulekile. Kuyasiza ukugcina i-PH yekhambi le-Assay futhi ihlinzeka ngokwenza izimo zokuphendula.
Ukuhlanzwa kwamaphrotheni:Ama-tes ajwayele ukusetshenziswa ezinhlelweni zokuhlanza amaprotheni ngenxa yokuthola amandla awo abubi nokuhambisana nemisebenzi ye-enzyme. Kuyasiza ukugcina ukuqina kanye nomsebenzi wamaprotheni ngesikhathi sokuhlanzwa kwezinyathelo.
I-electrophoresis:Ama-tes aqashwe njengengxenye yokuhlanza amasu we-gel electophoresures, ikakhulukazi ePoliyacryrylamide Gel Electrophoresis (ikhasi). Inikeza imvelo eqinile ye-PH yokuhlukaniswa kwamaprotheni nokufuduka.
Imidiya Yesiko Leseli:Ama-tes afakiwe kwabezindaba zesiko leselula ukuze alondoloze i-pH eqhubekayo ukukhula kweseli nokwanda. Kuyasiza ukuqinisa i-PH yesiko eliphakathi futhi kunikeze indawo efanelekile yamaseli.
Kubalulekile ukuqaphela ukuthi uhlelo lokusebenza oluthile kanye nokuhlushwa kwama-tes kungahluka ngokuya ngezidingo zokuhlola. Ukusingathwa okufanele, ukugcinwa, nokunaka imininingwane echazwe emibhalweni yezobuchwepheshe yomkhiqizo kubalulekile ukuqinisekisa ukusebenza kwayo nokuphepha kwayo.
